WY-14 643 rapidly activates nuclear factor kappaB in Kupffer cells before hepatocytes. (2024)

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Volume 19 Issue 7 Jul 1998

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Carcinogenesis, Volume 19, Issue 7, Jul 1998, Pages 1217–1222, https://doi.org/10.1093/carcin/19.7.1217

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01 July 1998

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    I Rusyn, H Tsukamoto, R G Thurman, WY-14 643 rapidly activates nuclear factor kappaB in Kupffer cells before hepatocytes., Carcinogenesis, Volume 19, Issue 7, Jul 1998, Pages 1217–1222, https://doi.org/10.1093/carcin/19.7.1217

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Abstract

Stimulation of cell proliferation caused by peroxisome proliferators was blocked by antibodies against TNF alpha and agents that inactivate Kupffer cells, a rich source of TNF alpha, which supports the hypothesis that Kupffer cells play a pivotal role in peroxisome proliferator-induced hyperplasia. Here, the ability of the very potent peroxisome proliferator WY-14 643 to activate the transcription factor NF-kappaB in rat liver was examined since it is involved in TNF alpha production. Female Sprague-Dawley rats were treated by gavage with WY-14 643 (100 mg/kg) while control animals were given equivalent doses of vehicle (olive oil). Activation of NF-kappaB in both whole liver, non-parenchymal cells, Kupffer cells and hepatocytes was assessed for up to 36 h using an electrophoretic mobility shift assay. In whole liver, WY-14 643 transiently increased NF-kappaB binding maximally 3.5-fold in 2-8 h followed by a steady decline to near control levels at 36 h. As early as 2 h after WY-14 643 treatment, the active form of NF-kappaB was localized predominantly in Kupffer cells with values 20- to 25-times greater than in hepatocytes. In hepatocytes, a small increase in NF-kappaB binding was observed but only 8 h after WY-14 643 administration. Pre-treatment with allopurinol, a xanthine oxidase inhibitor and free radical scavenger, suppressed NF-kappaB activation by WY-14 643 almost completely. It is concluded that NF-kappaB is activated by reactive oxygen species and plays a central role in the mechanism of action of peroxisome proliferators. Moreover, these findings support the hypothesis that Kupffer cells play a pivotal role in peroxisome proliferator-induced hepatocyte proliferation through rapid NF-kappaB activation and subsequent induction of TNF alpha production. TNF alpha from Kupffer cells stimulates growth in parenchymal cells later via mechanisms that also involve NF-kappaB.

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Topic:

  • olive oil
  • cell proliferation
  • allopurinol
  • electrophoretic mobility shift assay
  • free radical scavengers
  • hepatocytes
  • hyperplasia
  • kupffer cells
  • nf-kappa b
  • peroxisome proliferators
  • peroxisome
  • rats, sprague-dawley
  • reactive oxygen species
  • antibodies
  • liver
  • pharmaco*kinetics
  • rats
  • transcription factor
  • tube feeding
  • xanthine oxidase inhibitors
  • tumor necrosis factor-alpha

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